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. 2010 Oct;161(4):844–855. doi: 10.1111/j.1476-5381.2010.00911.x

Figure 1.

Figure 1

Figure 1

The NO and PG systems during the last part of pregnancy and during LPS-induced preterm labour (PTL). Female mice were killed at 10 h on days 13, 15, 16, 18 and 19 of pregnancy. Mice on day 15 of pregnancy treated with LPS (day 15 + LPS) (i.p., 10 µg at 10 h and 20 µg at 13 h) were killed at 22 h. Uterine strips were collected to measure: (A) Uterine production of prostaglandins E2 and F2α by RIA. aP < 0.001 versus all the rest, bP < 0.05 versus day 15. (B) Uterine NOS activity. aP < 0.001 versus days 13, 15 and 16; bP < 0.01 versus days 13, 15 and 16; cP < 0.001 versus day 15. (C) COX-2 and iNOS protein levels on LPS-induced PTL. Each blot was repeated three times with different samples. One representative blot is presented. aP < 0.001 versus day 15. (D) COX-1, eNOS and nNOS protein levels on LPS-induced PTL. Each blot was repeated three times with different samples. One representative blot of protein is presented. aP < 0.001 versus day 15. Values are expressed as means ± SEM (n ≥ 4). COX, cyclooxygenases; eNOS, endothelial nitric oxide synthase; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; nNOS, neuronal nitric oxide synthase; NO, nitric oxide; NOS, nitric oxide synthase; PG, prostaglandin.