Table 1.

Setup for preterm labour (PTL) model in mice

	Dose	n	PTL	TL	DM
A
One dose	80 µg	4	–	1	3
	60 µg	4	1	1	2
	50 µg	10	7	3	5a
	40 µg	4	–	2	2
	sham (1 dose)	4	–	4	–

B
Two doses	2 × 40 µg	4	–	–	4
	2 × 30 µg	4	–	–	4
	2 × 20 µg	6	6	–	2a
	1 × 10 µg + 1 × 20 µg	20	20 (100%)	–	1a
	2 × 10 µg	4	1	3	–
	sham (2 doses)	4	–	4	–

C
	Control	Sham	PTL
Beginning of partum	Day 19 morning	Day 19 morning	Day 15 nightb
Number of pups	10 ± 3	10 ± 3	9 ± 2

BALB/c female mice on day 15 of pregnancy were injected (A) once (at 10 h) or (B) twice (at 10 h and 13 h) with different doses of LPS (i.p.). Sham animals were treated with vehicle (physiological solution). n ≥ 4 for each treatment.

(C) Females on day 15 of pregnancy received no treatment (control) or were administered vehicle (physiological solution, sham group) or LPS twice (i.p., at 10 h and 13 h) and were observed up to the partum. Beginning of partum and number of pups were recorded. n≥ 4 for each treatment.

^aDuring preterm labour.

^b10 ± 2 h post LPS.

DM, dead mothers; PTL, preterm labour; TL, term labour.