Figure 4.

LXA₄ inhibits the zymosan-induced increase of preproET-1 mRNA expression and KC/CXCL1, LTB₄ and TNF-α production. Mice were treated with LXA₄ (20 ng per cavity) 1 h before the i.art. injection of zymosan (500 µg per cavity). After 2 h of zymosan stimulation, samples of the knee joints were collected for real-time PCR analysis (A) (n = 5 mice per group, each sample was analysed in triplicate). TNF-α (B), KC/CXCL1 (C) and LTB₄ (D) levels in knee synovial washes were determined 6 h after i.art. injection of zymosan (500 µg per cavity). Results are expressed as the mean ± SEM from at least six animals per group. *P≤ 0.05, significant differences between stimulated (zymosan) and non-stimulated groups; +P≤ 0.05, significant differences between treated (LXA₄) and untreated groups.