A. Cytokine release of PBMCs from different human donors after treatment with either recombinant or synthetic IDR-1. Cytokines tested are IL-6, TNFα as well as the chemokine MCP-1.
B. Purification table for IDR-1 showing the yield of pure IDR-1 after an initial pilot purification of 10 g of biomass from the fermentation as well as a projection of possible yields from processing the total biomass of this fermentation.
C. Tricine-SDS-PAGE comparing cleaved peptide yields of a high-density fermentation against a 1L shaking flask expression. The amounts shown for E5 and E6 are representative for all the peptides tested in shaking flasks expression.