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. Author manuscript; available in PMC: 2011 Nov 12.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Oct 14;402(2):421–424. doi: 10.1016/j.bbrc.2010.10.048

Figure 1.

Figure 1

Yeast cells containing the c-myc-sclerostin and HA-tagged "prey" protein expression are plasmids were grown for three days at 30 °C. Cells were lysed and immuno-precipitation was performed using c-myc (lanes A and D) or HA (lanes B and E) antibodies or no antibodies (lane C). The antibody complexes were captured by protein A beads, the protein precipitates were separated by SDS-PAGE and transferred onto PVDF membranes. The membranes were probed with c-myc or sclerostin monoclonal antibodies.