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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Mol Microbiol. 2009 Dec 4;75(2):452–461. doi: 10.1111/j.1365-2958.2009.06999.x

Figure 2. Association of DnaD, DnaB, DnaC (helicase) and Ssb depend on DnaA.

Figure 2

dnaAts mutant cells (WKS588) were grown at permissive temperature (30°C) and shifted to non-permissive temperature (52°C) for 1hr to inactivate DnaA and allow most ongoing rounds of replication to finish (light grey bars). Cells were then shifted to permissive temperature (35°C) in the presence of HPUra (dark grey bars) to allow replication to re-initiate and to trap initiation complexes at oriC. Indicated proteins were immunoprecipitated (IP’ed) from the cell lysates after crosslinking with formaldehyde. Error bars indicate the standard error of the mean (n=3). Note the different scale for the helicase IP on the right axis.