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. Author manuscript; available in PMC: 2010 Nov 29.
Published in final edited form as: Proteomics. 2009 May;9(10):2776–2787. doi: 10.1002/pmic.200800847

Figure 3. Analysis of the effects of SB203580 and PD98059 on RNF2.

Figure 3

A. Total protein extracts from plasmid control transfected HEK-293 cells were analyzed by 2D electrophoresis followed by western blotting using anti-RNF2 antibody as described under Materials and Methods. The region of the western blot in which RNF2 migrates was shown here. B. RNF2 was expressed transiently in HEK-293 cells and analyzed by 2D electrophoresis followed by western blotting using anti-RNF2. RNF2 was resolved into 7 protein spots, which are numbered for convenience. C. HEK-293 cells expressing RNF2 were treated with 20 µM SB203580 and then analyzed by 2D electrophoresis followed by western blotting using anti-RNF2. D. HEK-293 cells expressing RNF2 were treated with 50 µM PD98059 and then analyzed by 2D electrophoresis followed by western blotting using anti-RNF2.