Table 1.

List of tryptic peptides obtained from RNF2 and their modifications identified by nanoLC-MS/MS analysis.

Peptide #	Location in RNF2	Missed cleavage	Amino acid sequence	Theoretical Mass (mono)	Observed m/z	Charges	Experimental Mass	Delta	Modified residue	Mascot score
T1	2 – 15	0	acetyl-SQAVQTNGTQPLSK	1499.758	750.930	2	1499.860	0.102	S2	61
T1	2 – 15	0	acetyl-SQAVQTDGTQPLSK	1500.617	751.342	2	1500.684	0.067	S2, N7	82
T2	16 – 26	0	TWELSLYELQR	1436.730	719.381	2	1436.762	0.032		58
T3	27 – 43	0	TPQEAITDGLEIVVSPR	1823.963	913.026	2	1824.052	0.089		82
T3	27 – 43	0	TPQEAITDGLEIVVSPR	1823.963	609.031	3	1824.093	0.130		61
T3	27 – 43	0	TPQEAITDGLEIVVphosSPR	1903.929	952.918	2	1903.836	−0.093	S41	91
T3	27 – 43	0	TPQEAITDGLEIVVphosSPR	1903.929	635.757	3	1904.271	0.342	S41	52
T4	44 – 59	0	SLHSELMCPICLDMLK	1945.913	649.683	3	1946.049	0.064		58
T7	71 – 81	0	FCADCIITALR	1338.642	670.368	2	1338.736	0.096		69
T8/T9	82 – 91	1	SGNKECPTCR	1207.507	604.793	2	1207.586	0.079		53
T13/T14	98 – 112	1	RSLRPDPNFDALISK	1727.932	577.029	3	1728.027	0.095		57
T14	99 – 112	0	SLRPDPNFDALISK	1571.831	786.972	2	1571.944	0.113		60
T15/T16	113 – 126	1	IYPSRDEYEAHQER	1791.818	896.914	2	1791.828	0.01		59
T16	118 – 126	0	DEYEAHQER	1175.484	588.782	2	1175.564	0.08		44
T18/T19	131 – 149	1	INKHNNQQALSHSIEEGLK	2159.108	720.780	3	2159.340	0.232		53
T19	134 – 149	0	HNNQQALSHSIEEGLK	1803.886	902.929	2	1803.858	−0.072		98
T24/T25	162 – 196	1	QQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKR	3669.533	918.363	4	3669.452	−0.081		56
T27	199 – 239	0	TSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEK	4442.072	1111.706	4	4442.824	0.752		37
T30	250 – 261	0	TSGNATVDHLSK	1228.605	410.635	3	1228.905	0.300		40
T32	267 – 273	0	LALEELR	842.486	422.281	2	842.562	0.076		49
T33/T34	274 – 289	1	SKGESNQMNLDTASEK	1737.784	869.847	2	1737.694	−0.090		121
T34	276 – 289	0	GESNQMNLDTASEK	1522.657	762.375	2	1522.750	0.093		79
T37	321 – 333	0	VNKPMELYYAPTK	1552.796	777.478	2	1552.956	0.150		70
T37/T38	321 – 336	1	VNKPMELYYAPTKEHK	1946.992	650.086	3	1947.258	0.260		46