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. Author manuscript; available in PMC: 2010 Nov 29.
Published in final edited form as: Development. 2006 Nov 8;133(24):4815–4825. doi: 10.1242/dev.02664

Fig. 2. Expression of Bhlhb5 in GABAergic amacrine and OFF-cone bipolar subtypes.

Fig. 2

Sections from P28 mouse retinas were double-immunolabeled with anti-Bhlhb5 (red) and subtype-specific markers (green) as indicated. (A–C) The Bhlhb5+ cells in the GCL and the ACL are Pax6+ amacrine cells. (D–F) Bhlhb5 is expressed in GAD65+ GABAergic amacrine cells. (G–I) Bhlhb5 is co-expressed with Prox1 in displaced amacrine cells (arrows) in the GCL and bipolar cells (arrowheads) in the INL. (J–L) Bhlhb5 is co-expressed with Chx10 in bipolar cells (arrowheads). Inserts show the double-labeling of bipolar cells (asterisks) at high magnification. (M–O) All Bhlhb5+ bipolar cells are Vsx1+ cone bipolar cells (white arrowheads). (P–R) All Bhlhb5+ bipolar cells (white arrowheads) are recoverin+ Type 2 OFF-bipolar cells; red arrowheads indicate the OFF-bipolar cells immunoreactive to recoverin only. Scale bar: 50 μm.