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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Neuroscience. 2010 Sep 20;171(2):588–598. doi: 10.1016/j.neuroscience.2010.08.056

Figure 5.

Figure 5

Early, but not late administration of NGF sequestering therapy reduces sarcoma-induced nerve sprouting of CGRP+, NF200+, and TH+ nerve fibers. At day 20 post cell injection, the density of CGRP+ (A), NF200+ (B), and TH+ (C) nerve fibers is significantly greater in sarcoma + vehicle-treated mice compared to sham + vehicle-treated mice. This tumor-induced nerve sprouting is significantly attenuated by early/sustained (10 mg/kg; i.p., given at days 6, 12, and 18 post cell injection), but not by late/acute (10 mg/kg; i.p., given at day 18 post cell injection) administration of anti-NGF. Nerve fiber density was determined by measuring the total length of nerve fibers per unit area in the periosteum. Confocal images from three decalcified bone sections per animal and per marker were acquired in the proximal metaphyseal periosteum (∼2 mm below the growth plate) and used to determine the length of nerve fibers. Both imaging acquisition and analysis were performed in a blinded fashion. Brackets indicate the groups being compared. *p<0.05, **p<0.01, ***p<0.001. Bars represent the mean ± SEM. The number of animals was n=8 for sham, n=9 for sarcoma + vehicle, n=9 for sarcoma + early/sustained anti-NGF, and n=7 for sarcoma + late/acute anti-NGF.