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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Neurochem. 2010 Apr 9;114(1):97–109. doi: 10.1111/j.1471-4159.2010.06744.x

Figure 4.

Figure 4

NPC/GPCs express both CCR5 and CXCR4 in vitro. Olig2+ (green) oligodendroglial precursors show both CCR5 (A) and CXCR4 (C) immunoreactivity. The signal for CXCR4 was generally light in these cells, and is indicated by arrows in (C). Undifferentiated Sox2+ NPC/GPCs (green) also are immuno-positive for CCR5 (B) and CXCR4 (D). Asterisks in A-D indicate double-labeled cells. Hoechst 33342 nuclear staining (blue) shows that some cells positive for CCR5 or CXCR4 do not express Olig2 or Sox2. (E) A major band at approximately 40 kD was noted on immunoblots of NPC/GPCs harvested at 5 DIV using antibodies to both CCR5 and CXCR4. Actin detection is included as a loading control. These bands are also detected in homogenates of cultured astroglia (AS) and whole striatum, preparations that are known to express both receptors.