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A fluorescent viability assay reveals that 12h exposure to HIV-1 Tat1-86 or gp120 alone or in combination with opiates did not increase the rate of NPC/GPC cell death in vitro. Cells were treated at 5 days after plating with morphine (M), Tat (T), gp120 (G), naloxone (N) either alone or in the combinations indicated. There was no significant increase in cell death in any treatment group. N=8 independent cultures.
