Table 1.
Multiplex analysis of chemokines/cytokines secreted by E15 striatal progenitors (pg/ml)
IL-1β | GM-CSF | TNF-α | IL-6 | IL-9 | KC | RANTES | MIP-1α | MIP-1β | MCP-1 | |
---|---|---|---|---|---|---|---|---|---|---|
C | <0.85# | <1.17# | <0.3# | 1.78±1.06 | <20.4# | 6.70±2.42 | 5.74±0.56 | 57.86±15.59 | 135.5±36.09 | 68.77±26.9 |
M | 1.10±0.25 | <1.17# | <0.3# | <0.72# | 23.21±2.57 | 6.21±2.52 | 5.09±0.35 | 26.94±0.02 | 98.07±22.08 | 57.03±24.12 |
T | 5.54±1.52 | 2.43±0.83 | 0.68±0.28 | 5.10±2.88 | 28.85±3.15 | 25.40±10.81 | 336±26.8 | 312.5±25.11 | 957±102.5 | 183.6±78.2 |
G | 1.35±0.32 | 1.64±0.47 | 0.44±0.09 | <0.72# | 27.54±6.53 | 7.63±2.29 | 6.34±1.03 | 119.5±62.8 | 273±185.1 | 94.26±38.65 |
N | <0.85# | <1.17# | 1.27±0.21 | <0.72# | <20.4# | 6.11±2.39 | 3.59±0.81 | 26.95±0.0 | 75.17±16.64 | 58.83±23.72 |
MT | 5.76±2.31 | 1.64±0.47 | 1.14±0.36 | 4.81±3.00 | 26.03±3.15 | 20.61±9.52 | 292±59.7 | 307.1±62.06 | 714.5±127.8 | 170.15±76.58 |
MG | <0.85# | 1.96±0.79 | 0.34±0.04 | <0.72# | <20.4# | 5.74±2.03 | 5.06±1.09 | 48.63±21.69 | 94.12±17.35 | 47.85±16.08 |
MTN | 7.46±4.47 | 2.46±1.29 | 0.76±0.23 | 6.72±4.74 | 23.21±2.57 | 32.62±15.89 | 397±105 | 376.8±104.6 | 1009.1±194 | 237.2±131.6 |
MTG | 6.99±2.77 | 3.22±0.96 | 1.20±0.81 | 3.09±1.06 | 26.03±3.15 | 24.12±9.13 | 271.7±32.33 | 321.9±61.6 | 945±165.9 | 167.9±75.83 |
MTGN | 9.87±4.02 | 1.81±0.64 | 0.51±0.14 | 1.78±1.61 | <20.4# | 24.81±9.51 | 336.3±28.31 | 376.0±46.9 | 1063±116.3 | 124.8±75.26 |
MN | <0.85# | <1.17# | <0.3# | <0.72# | <20.4# | 5.85±2.58 | 4.08±0.58 | 26.94±0.0 | 66.93±18.06 | 55.79±21.8 |
C=control; M=morphine; Tat=Tat; G=gp120; N=naloxone.
The following proteins were not routinely detected in control or treated cells: IL1α, IL-2, IL-3, IL-4, IL-5, IL-10, IL-12(p40), IL-12(p70), IL-13, IL-17, G-CSF, Eotaxin, IFNγ
< x.xx designates that sample concentration was determined to be below the accuracy limit of the assay software, based on the standard curve generated for the individual chemokine/cytokine. In those cases, we have indicated the lower limit value in pg/ml. For all samples, N=6.