Figure S2.

RP-HPLC separation of the analytes in the apoptotogenic aqueous extract of cyanobacterial strain M44. The sample (60 μL) prepurified by solid phase extraction (see above) was further purified by chromtaography on a column (Aquasil C18; 3 × 150 mm, Thermo Hypersil-Keystone) coupled to a Merck-Hitachi LaChrom HPLC system (VWR, West Chester, USA). The mobile phases were 10mM TEAF pH 3 (A), and 100% methanol (B). The flow rate was 0.5 mL/min and monitoring wavelength was 250nm. The bioactivity eluted in fraction 2.–3.8 min (red).