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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Nucl Med Biol. 2010 Nov 1;37(8):917–925. doi: 10.1016/j.nucmedbio.2010.05.001

Fig. 2.

Fig. 2

Fig. 2

HPLC profile of a [18F]AV-45 ([18F]5) sample purified by SPE method (trace A: radio trace of sample; trace B: UV trace of sample @ 350 nm). Trace C is an UV trace of non - radioactive reference compound AV-45 (@350 nm). The bottom trace (D) is an UV trace of AV-136 (6) (@350 nm).

HPLC profile of [18F]AV-45 ([18F]5) sample purified by SPE method (HPLC conditions: Agilent HPLC 1100 ; Gemini C18 column (250mm × 4.6 mm); mobile phase: 1 mL/min with a gradient as follows: from 0 – 2 min isocratic 10 mM ammonium formate buffer (AFB) 100%; from 2 - 5min gradient AFB 100 - 30%, ACN 0 - 70%; from 5 - 10 min gradient AFB 30 - 0%, ACN 70 - 100% and from 10 - 15 min AFB 100%).

A: radioactive trace of sample

B: UV trace of sample @ 350 nm

C: UV trace of cold AV-45 ([19F]5) under identical HPLC conditions as above.

D: UV trace of synthesized AV-136 @ 350 nm under identical HPLC conditions as above. The “identical” retention times of AV-136 compared to the UV material of the labeled sample suggest that the byproduct in the labeling is AV-136 (6).