FIG. 2.

Generation of human iPSCs with feeder-independent, serum-free condition with defined medium. (A) Outline of experimental design. Freshly isolated human dermal fibroblasts in mTeSR™1 medium supplemented with 50 ng/mL bFGF were transduced with lentiviruses. Seven days posttransduction, ROCK inhibitor Y-27632 was applied. (B) Colonies of human iPSCs (arrowheads) in the background of incomplete reprogrammed cells 28 days posttransduction. (C) Hand-picked putative human iPSC colony subcultured to new Matrigel™-coated wells. (D–F) Morphology of hand-picked human iPSC colony 5 days (D), 10 days (E) after subculture, and at 10 passages. (G) Intensive alkaline phosphatase expression in human iPSC colony (purple color).