Table 1.
PCR Primers and Conditions of Semiquantitative RT-PCR
| Gene | Accession no. | Forward/reverse primer sequence (5′-3′) | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|---|
| RE | NM174900.3 | GCGTACGCAAATTAAAGTCCAGA | 58 | 260 |
| X1 | CAGCATCCTAAACAGCTCGCAGAAT | |||
| Nestin | NM006617.1 | GGCAGCGTTGGAACAGAGGTTGGA | 58 | 694 |
| CTCTAAACTGGAGTGGTCAGGGCT | ||||
| ICAM | NM000201.2 | ACCATGGAGCCAATTTCTC | 58 | 673 |
| ACAATCCCTCTCGTCCAG | ||||
| IHH | NM002181.2 | CTACGCCCCGCTCACAAAG | 58 | 375 |
| GGCAGAGGAGATGGCAGGAG | ||||
| AFP | NM001134.1 | GGGAGCGGCTGACATTATTA | 58 | 346 |
| CCCTCTTCAGCAAAGCAGAC | ||||
| GAPDH | NM002046 | AGCCACATCGCTCAGACACC | 58 | 308 |
| GTACTCAGCGGCCAGCATCG |
REX1, RNA exonuclease 1; ICAM, intercellular adhesion molecule 1; IHH, Indian hedgehog homolog; AFP, alpha-fetoprotein; GAPDH, glyceraldehde 3-phosphate dehydrogenase (Xu et al., 2001).