Figure 4.

Effects of FTY720 and AUY954 on BAL leukocytes after bleomycin challenge. (A–C) FTY720 treatment had no effect on total leukocyte accumulation in the airspaces after bleomycin lung injury, although a decrease in BAL T cells occurred, as well as an increase in BAL neutrophils, in FTY720-treated compared with vehicle-treated mice. (D–F) Similar effects on BAL leukocyte accumulation were evident with AUY954 treatment, with no difference in total leukocytes and trends toward decreased T cells and increased neutrophils. Data are presented as means ±SEM, with n = 5 mice/group. *P < 0.05, **P < 0.005, and ***P < 0.0005, comparing FTY720-treated or AUY954-treated versus vehicle-treated groups at each time point.