Effects of FTY720 and AUY954 on BAL leukocytes after bleomycin challenge. (A–C) FTY720 treatment had no effect on total leukocyte accumulation in the airspaces after bleomycin lung injury, although a decrease in BAL T cells occurred, as well as an increase in BAL neutrophils, in FTY720-treated compared with vehicle-treated mice. (D–F) Similar effects on BAL leukocyte accumulation were evident with AUY954 treatment, with no difference in total leukocytes and trends toward decreased T cells and increased neutrophils. Data are presented as means ±SEM, with n = 5 mice/group. *P < 0.05, **P < 0.005, and ***P < 0.0005, comparing FTY720-treated or AUY954-treated versus vehicle-treated groups at each time point.