Figure 5.

Activity of eNOS (A) and NOS (B) in lung tissue of rats, and Western blotting for eNOS and β-actin expression in MCT-treated rat lungs (C and D). A significant decrease occurred in eNOS activity (A) and expression (C and D) in rats treated with MCT, either alone or together with pCB6 empty vector. In contrast, in MCT-treated rats receiving pCB6-2J2, eNOS activity and protein levels were near normal. l-NAME significantly weakened the CYP2J2-induced increase in eNOS activity. Results are representative of 3–5 independent experiments. (D) Blots were scanned, and relative eNOS expression was normalized to β-actin . Data shown are mean ± SEM of 6–8 rats/group. *P < 0.05 versus control. ^#P < 0.05 MCT + CYP2J2 versus MCT + pCB6. ^&P < 0.05 MCT + CYP2J2 + l-NAME versus MCT + CYP2J2. ^{^}P < 0.05 MCT + CYP2J2 + l-NAME versus MCT + l-NAME. ^@P < 0.05 MCT + l-NAME versus MCT