Table I.
PAL tested | Km (µM) | Relative Vmax (pmol/h/OD) |
---|---|---|
PALcc | 13.8 ± 2.8 | 1.00 ± 2.8 |
R533A | 21.3b | 0.03 ± 0.006a |
R533Q | 24.2b | 0.02 ± 0.007a |
R706A | 25.5b | 0.03 ± 0.006a |
R706Q | 15.8 ± 1.5a | 0.26 ± 0.039a |
M784A | 95.2b | 0.41 ± 0.005a |
M784Q | 94.6 ± 21.6a | 0.39 ± 0.014a |
D787A | 33.4 ± 5.5 a | 1.19 ± 0.16 |
significantly different from PALcc, p<0.01
determined once