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. Author manuscript; available in PMC: 2010 Nov 29.
Published in final edited form as: Structure. 2009 Jul 15;17(7):965–973. doi: 10.1016/j.str.2009.05.008

Table I.

Kinetic Analysis of PALcc mutants

PAL tested Km (µM) Relative Vmax (pmol/h/OD)
PALcc 13.8 ± 2.8 1.00 ± 2.8
R533A 21.3b 0.03 ± 0.006a
R533Q 24.2b 0.02 ± 0.007a
R706A 25.5b 0.03 ± 0.006a
R706Q 15.8 ± 1.5a 0.26 ± 0.039a
M784A 95.2b 0.41 ± 0.005a
M784Q 94.6 ± 21.6a 0.39 ± 0.014a
D787A 33.4 ± 5.5 a 1.19 ± 0.16
a

significantly different from PALcc, p<0.01

b

determined once