Fig. 5.

Relative levels of expression of mRNA for various proteins in the small intestine of mice deficient in both ABCA1 and ACAT2. All the mice were females fed a basal rodent chow diet. Relative mRNA levels were determined by expressing the amount of mRNA found relative to that for the Acat2^+/+/Abca1^+/+ mice that in each case was arbitrarily set at 1.0. In B the open bars for the 2 groups lacking ABCA1 reveal that the PCR primers utilized for this assay detect an aberrant transcript that does not produce functional ABCA1 protein. In all panels values are means ± SE of data from a minimum of 5–7 mice per group. One-way ANOVA revealed significant differences among groups (A–E) and bars designated with different letters denote statistically different values, P < 0.05.