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. 2010 Aug 26;299(5):G1087–G1096. doi: 10.1152/ajpgi.00124.2010

Fig. 4.

Fig. 4.

Effects of L. reuteri strains on LPS-induced IL-13 levels in rat intestine. A: IL-13 mRNA expression levels from the ilea of rat pups with L. reuteri strains and LPS feeding. qRT-PCR analysis was performed to determine the expression level (fold changes) of IL-13 comparing L. reuteri + LPS-fed rats with LPS-fed rats. Data are represented as means ± SE, N = 6. Dotted lines show cutoff values of fold change: > +2 indicating upregulation, < −2 downregulation. *P < 0.05 vs. LPS control. L. reuteri strains DSM 17938 and ATCC PTA 4659 significantly downregulated mRNA expression of IL-13 induced by LPS. B: IL-13 protein level in rat intestine. IL-13 protein in ileal tissue lysates were assayed by using a MSD rat multiplex assay. Data are represented as means ± SE, N = 8–10. Formula ± LPS-fed groups compared with dam-fed control: **P < 0.01. Formula + L. reuteri groups compared with formula-fed group: $P < 0.05. L. reuteri + LPS groups compared with LPS group: #P < 0.05. L. reuteri strains DSM 17938 and ATCC PTA 4659 inhibit LPS-induced IL-13 production. Only L. reuteri strain DSM 17938 significantly reduced formula-stimulated IL-13.