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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Int J Cancer. 2010 Jun 1;126(11):2622–2634. doi: 10.1002/ijc.24957

Figure 2. NKT cells and MvP728 synergistically activate DCs.

Figure 2

(A) Human monocyte-derived immature DCs were infected with MvP728 at indicated MOI. Extracellular bacteria were killed by gentamicin after 1 h. Human in vitro expanded NKT cells (1:1 NKT:DC ratio) and GSL1 (200 ng/ml) were added to culture when indicated. After 48 h, DC phenotype was analyzed by FACS. (B) IL-12 concentration in supernatants in the same experiments was determined by CBAPlex. Results are means ± SD from 4 experiments. * P<0.05, **P<0.01, ***P<0.001, one-way ANOVA.