Fig. 4.

TUNEL-positive nuclei (A) and levels of the active form of caspase-3 (B) and calpain (C) proteins in cardiac tissue of sedentary, exercise, sedentary-Dox, and exercise-Dox animals. Representative images of immunostained cryosections from cardiac tissue are shown. Sections were stained for nuclei (blue), laminin (red), and TUNEL-positive nuclei (green). Western blots of the active band of caspase-3 and calpain are shown above the graphs. Data are presented as means ± SD. Sedentary, n = 7; exercise, n = 6; sedentary-Dox, n = 5; and exercise + Dox, n = 6. *P < 0.05 compared with sedentary, exercise, and exercise-Dox; †P < 0.05 compared with sedentary and exercise.