Fig. 6.

PKG-I modified Elk-1 inhibition of myocardin-induced SM22 promoter activity. COS-7 cells were transiently transfected with Flag-Elk-1 (25 ng), myocardin (25 ng), or both with or without PKG-Iα (50 ng)-expressing plasmids. SM22 promoter-driven firefly luciferase activities were normalized with the cotransfected renilla luciferase activity. Relative luciferase activity is expressed as fold activation above the level of expression of the reporter gene alone. Relative levels of protein expression were shown by immunoblots. Experiment was repeated 3 times in triplicate, and the data presented are from a representative experiment. *P < 0.05 compared with Elk-suppressed Myocd-induced SM22 promoter activity.