Fig. 8.

Abolition of EDHF-mediated responses of uterine arteries by charybdotoxin (CTX) and apamin (AP). A–D: bar graphs demonstrating inhibition of ACh-induced SMC [Ca²⁺]_i and dilator responses by a combined treatment of arteries with 50 nM CTX and 100 nM AP. Both toxins were applied intra- and extraluminally. Arteries were preconstricted with PE before testing ACh. l-NNA (200 μM) and indomethacin (10 μM) were present throughout the whole experiment. ACh-induced vasodilation is expressed as D_max. Nos. in parentheses indicate the no. of tested arteries. *Significantly different at P < 0.05 (2-way repeated-measures ANOVA).