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. 2010 Sep 10;299(5):H1374–H1381. doi: 10.1152/ajpheart.00168.2010

Fig. 4.

Fig. 4.

The effect of poly(ADP-ribose) polymerase (PARP) inhibition on AIF release 10 days after Dox injection with or without 4-amino-1,8-naphthalimide (4-AN). A: PARP activity assay in WT and CrmA Tg mice 10 days after Dox injection. *P < 0.05; n = 4 mice. B: caspase-3-like activities assay in WT and CrmA Tg mice 10 days after Dox injection. *P < 0.05; n = 4 mice. C: representative Western immunoblots of AIF in the mitochondria-free, cytosolic fraction of WT or CrmA heart after Veh, Dox, or Dox + 4-AN administration. GAPDH was used as an internal control. The 2 rightmost columns of blots represented the Dox + 4-AN conditions for the WT and CrmA groups. D: quantification of cytosolic protein content of AIF. *P < 0.05; n = 4 mice. E: AIF immunofluorescent staining of WT and CrmA Tg mice 5 days after Dox injection with or without 4-AN. Arrows indicate dual staining for AIF (green) and nuclei (blue), suggesting AIF translocation to the nuclei. AIF, green; nuclei, blue; α-actinin, red. Magnification = ×60.