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. 2010 Sep 10;299(5):H1715–H1727. doi: 10.1152/ajpheart.00337.2010

Fig. 3.

Fig. 3.

Effect of NAG-thiazoline treatment on O-linked β-N-acetylglucosamine (O-GlcNAc) levels of the perfused rat heart. Representative anti-O-GlcNAc (CTD110.6) immunoblot (A) and densitometric results of protein-associated cardiac O-GlcNAc levels (B) after time-control, normoxic perfusions (Norm); and after 20 min zero-flow ischemia and 60 min reperfusion (I/R) in the absence (control) and in the presence of NBt50, NBt100, and NAe treatments. Densitometric analyses were performed using original anti-O-GlcNAc immunoblots to compare all experimental groups (n = 3–5 hearts/group). The entire lane mean intensities are normalized to calsequestrin levels shown as protein loading control, and are relative to the control group. P < 0.001 vs. control (*), vs. NBt50 (†), vs. NBt100 (‡), and vs. Norm (§).