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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Immunol. 2010 Feb 14;11(3):240–249. doi: 10.1038/ni.1845

Figure 2.

Figure 2

The defect in iNKT cell development by HEB-TKO thymocytes is cell intrinsic. (a) Expression of CD45.1 and CD1d-tet by donor thymocytes (left) and expression of CD1d-tet and CD24 by total thymocytes and wild-type (CD45.1+) and HEB-TKO thymocytes in reconstituted recipients (right). Data are representative of two experiments. (b) Surface expression of NK1.1 and CD44 by CD1d-tet+ donor cells from thymus and spleen of bone marrow chimeras. Data are representative of two experiments with five chimeras total. Numbers adjacent to outlined areas or in quadrants (a,b) indicate percent cells in each. (c) Total CD1d-tet+TCRβ+ cells among wild-type or HEB-TKO donor cell populations from thymus, spleen and liver. Numbers above HEB-TKO indicate average number of cells (bars not visible). Data are representative of QQ experiments (average and s.e.m. of five mice). (d) CD1d surface expression (black lines) by thymocytes and splenocytes from wild-type, E2A-TKO, HEB-TKO and E2A-HEB-TKO mice. Gray-filled histograms, unstained cells. Numbers in plots indicate mean fluorescence intensity. Data are representative of three independent experiments.