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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cell. 2010 Sep 16;143(1):71–83. doi: 10.1016/j.cell.2010.08.038

TABLE 1.

Non-synonymous Genetic Changes in the Evolved Disomic Strains

STRAIN1 GENE MUTATION METHOD2 PROTEIN FUNCTION
Dis V-14.1 SNT1 L431R S, T Subunit of the Set3C deacetylase complex
RAD3 (het) D148N S, T 5′ to 3′ DNA helicase, involved in nucleotide excision repair
UBP63 E256X S, T Ubiquitin-specific protease
DYN1 L526R S Cytoplasmic dynein heavy chain
TSL1 N127D S Subunit of trehalose 6-phosphate synthase
Chr X, 31906 C to G S, T Intergenic region
Chr XIII, 442441 A to C S, T Intergenic region
Dis VIII-14.1 RPT1 Q281K T ATPase part of the 19S regulatory particle of the proteasome
Chr V, 140399 C to G T Intergenic region
Dis IX-14.1 VPS643 Q23X T Vacuole targeting factor
UBP63 E404X T Ubiquitin-specific protease
Dis XI-9.1 VPS643 E103G S Vacuole targeting factor
SRC1 I721V S Inner nuclear membrane protein
Chr IX, 338123 C to T S Intergenic region
Chr XIII, 818616 G to T S Intergenic region
Dis XI-9.2 SAS10 G311V S Subunit of processome complex
RSP54 V591M S, T Ubiquitin-protein ligase
Chr IX, 1836144 G to A S, T Intergenic region
Dis XI-14.1 RSP54 V591M S, T Ubiquitin-protein ligase
Chr IX, 1836144 G to A S, T Intergenic region
Dis XIV-9.1 YGR266W D450Y S Protein of unknown function
Chr VII, 827547 C to T S Intergenic region
LAG24 D644E S Protein involved in determining longevity
YNL234W4 D16N S Heme-binding protein involved in glucose signaling
Chr XIV, 6230234 C to S S Intergenic region
Dis XIV-9.2 UBR1 F951C S Ubiquitin-protein ligase
DCS2 H269Y S Stress induced protein
CCT7 P114R S Subunit of the chaperonin Cct ring complex
Chr XIV, 148095 A to W S Intergenic region
LAG24 D644E S Protein involved in determining of longevity
YNL234W4 D16N S Heme-binding protein involved in glucose signaling
Chr XIV, 6230234 C to S S Intergenic region
Dis XIV-14.2 PRR2 E260X T Serine/threonine protein kinase
BUD9 E499D T Protein involved in bud-site selection
Chr XVI, 572683 C to G T Intergenic region
Dis XVI-9.1 SAS3 S689R S Histone acetyltransferase catalytic subunit of NuA3 complex
SVF15 W178X S Protein with a potential role in cell survival pathways
Dis XVI-14.1 SEC31 S1116T S Essential component of the COPII coat of secretory pathway vesicles
UTP10 P173S S Subunit of processome complex involved in production of 18S rRNA
SVF15 A320P S Protein with a potential role in cell survival pathways
Dis XVI-14.2 GRX4 F188L S Glutathione-dependent oxidoreductase
SVF15 E220X S Protein with a potential role in cell survival pathways
Chr I, 71729 T to C S Intergenic region
1

9.1 and 9.2 refer to isolates 1 and 2 from day 9, respectively. 14.1 and 14.2 refer to isolates 1 and 2 from day 14, respectively.

2

S = Solexa sequencing, T = Tiling arrays.

3

This gene is mutated in descendants of different disomes.

4

This mutation is present in more than one isolate.

5

3 different mutations of SVF1 are present in 3 isolates of disome XVI.