Figure 8.

Inhibiting survivin signaling via inactivation of survivin by siRNA. A: When siRNA against survivin was transfected into HT29 cells, survivin mRNA expression decreased as determined by RT-PCR (Lane 1 = nontargeting siRNA; Lane 2 = survivin siRNA; Lane 3 = transfection reagent only; Lane 4 = cyclophilin B siRNA). B and C: In the same cells, a similar decrease was observed for survivin protein and endogenous phospho-histone-H3 protein levels as determined by Western blot analysis. Densitometry showed that for survivin, the RNA level decreased by 68%, survivin protein by 61%, and endogenous phospho-histone-H3 levels by 66% after anti-survivin siRNA was transfected into HT29 cells.