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Quantitative assessment of complement deposition post-SCI. The deposition of C3 (C3 days), fB, and C9 (MAC) was analyzed at 24 hours (A) and 72 hours (B) after injury in spinal cord sections from the epicenter of injury. Mice received anti-fB mAb or PBS (vehicle) at 1 and 12 hours after injury. Analysis was performed using immunofluorescence microscopy, and sections scored for fluorescence intensity on a 4 point scale (see Materials and Methods). Mean ± SD, n = 6. *P < 0.01.
