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. 2010 Dec;177(6):2898–2911. doi: 10.2353/ajpath.2010.100367

Figure 1.

Figure 1

Kinetic analyses of visceral L. infantum infection. A: Cells were infected with promastigotes at a 10:1 (parasites/cell) ratio for 4 hours. BMDCs were immediately recovered or left in culture for an additional 12, 24, 48, or 72 hours. A fluorescent nuclear label, 4′,6-diamidino-2-phenylindole, was added to the cells, and the number of infected cells as well as the number of parasite per cell was counted with a fluorescence microscope at ×1000. Results are representative of three independent experiments. B: BMDCs infected with GFP-L. infantum promastigotes at a 10:1 (parasites/cell) ratio were recovered after 4 hours of incubation or left in culture for an additional 24 hours after the end of the infection. The cells were analyzed by flow cytometry, and the percentage of GFP-positive BMDCs was quantified. Each value represents the mean ± SD from three independent experiments. NS, not significant; *P < 0.05.