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. 2010 Dec;177(6):2984–2999. doi: 10.2353/ajpath.2010.100447

Figure 5.

Figure 5

LAMP1-positive storage vesicles express GM130 in the MPSIIIB mouse brain. A: Parasagittal rostral cortex sections of 8-month-old wild-type or MPSIIIB mice were immunolabeled for LAMP1 and GM130. Confocal immunofluorescence (LAMP1 in purple, GM130 in green, nuclei counterstained in blue) shows frequent colocalization of LAMP1 and GM130 signals (in white) in MPSIIIB cortical sections. Scale bars, 10 μm. B–H: Immunogold electron microscopy was performed on ultra-thin cryosections of the rostral cortex of 8-month-old wild-type (B) or MPSIIIB (C–H) mice. Sections were labeled with anti-GM130 antibodies revealed with 10-nm gold particles alone or in combination with anti-LAMP1 antibodies revealed with 15-nm gold particles. Golgi structures decorated with GM13010 nm particles are shown (B and C). Distended vesicles in which the limiting membrane is doubly labeled with LAMP115 nm and GM13010 nm were frequently observed in MPSIIIB neurons (D–H). GC, Golgi complex. Scale bars, 0.2 μm.