Figure 3.
Effect of WIN 55212-2 on ROS production. Productions of O2·− (A), H2O2 (B), and NO (C) in each stromal endometriotic cell line (11 eutopic endometrial stromal cell lines, Es, and 14 deep infiltrating endometriotic stromal cell lines, Ds) treated in vitro with increasing concentrations (0.3 to 40 μmol/L) of WIN 55212-2 compared to untreated cells. Black dotted line shows the basal level of production in untreated cells. Production of ROS was assessed by spectrofluorometry using specific fluorochromes. Results are expressed as ratios of treated versus untreated cells: *P < 0.05.