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. 2010 Dec;177(6):2791–2803. doi: 10.2353/ajpath.2010.100129

Figure 1.

Figure 1

ANGPTL4 expression is elevated in wound biopsies. Expression profiles of ANGPTL4 (A) mRNA and (B) protein during wound healing determined by qPCR and immunoblotting, respectively. Ribosomal protein L27 was used as a normalizing reference gene. Polyclonal antibodies that recognized the N (anti-nANGPTL4) and C termini (anti-cANGPTL4) of ANGPTL4 were used. β-tubulin was used as loading and transfer control. Values at each time point are mean ± SEM of 15 mice. C: Immunofluorescence staining of ANGPTL4 in wound biopsies. Mouse skin wound biopsies at indicated days of postwounding were cryosectioned, stained for cANGPTL4 and Ki-67, and counterstained with DAPI. Representative pictures from the wound edge and adjacent wound bed are shown. Arrow denotes the wound edge at day 0. Dotted white line represents epidermal-dermal junction. Scale bar = 40 μm. D: Relative expression levels of ANGPTL4 mRNA in normal human skin biopsies and ulcers determined by qPCR. Ribosomal protein L27 was used as a reference gene. Each circle shows the mean values of three different paraffin sections from an individual sample; horizontal bars show average values obtained from human skin biopsy or ulcers.