Table 1.

Oligonucleotide Sequences of siRNA and Real-Time PCR Primers Used in this Work

Oligonucleotide	Sequence
siRNA
ANGPTL4 siRNA
Sense	5′-AAAGCTGCAAGATGACCTCAGATGGAGGCTG-3′
Anti-sense	5′-AAAAGGCTTAAGAAGGGAATCTTCTGGAAGAC-3′
Control siRNA
Sense	5′-AAAGCTGTCTTCAAGATTGATATCGAAGACTA-3′
Anti-sense	5′-AAAATAGTCTTCGATATCAAGCTTGAAGACA-3′
Real-time qPCR*
Human ANGPTL4
Forward	5′-CTCCCGTTAGCCCCTGAGAG-3′
Reverse	5′-AGGTGCTGCTTCTCCAGGTG-3′
Taqman probe	5′-(6-FAM)ACCCTGAGGTCCTTCACAGCCTGC(TAMRA)-3′
Mouse ANGPTL4
Forward	5′-GCTTTGCATCCTGGGACGAG-3′
Reverse	5′-CCCTGACAAGCGTTACCACAG-3′
Taqman probe	5′-(6-FAM)ACTTGCTGGCTCACGGGCTGCTAC(TAMRA)-3′
L27
Forward	5′-CTGGTGGCTGGAATTGACCGCTA-3′
Reverse	5′-CAAGGGGATATCCACAGAGTACCTTG-3′
Taqman probe	5′-(HEX)CTGCCATGGGCAAGAAGAAGATCGCC(BHQ1)-3′

^*Melting curve analysis was performed to assure that only one PCR product was formed. Primers were designed to generate a PCR amplification product of 100 to 250 bp. Only primer pairs yielding unique amplification products without primer dimer formation were subsequently used for real-time PCR assays.