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. 2010 Dec;177(6):2701–2707. doi: 10.2353/ajpath.2010.100617

Figure 1.

Figure 1

Overexpression of phospho-IGF-IR and phospho-AKT in malignant gastric tissue. A: Immunohistochemical staining showing overexpression of phospho-IGF-IR in the majority of the tumor cells in the invasive malignant glands in the deeper region of the mucosa as indicated by (T) compared with the adjacent superficial nonmalignant glands (N). Original magnification, ×40. B: Tumor cells are strongly positive for phospho-IGF-IR antibody showing intense membrane and cytoplasmic staining indicated by arrows as shown in higher magnification (×300). D and F: Immunoprecipitation followed by immunoblot shows phospho-IGF-IR and total IGF-IR expression levels were significantly higher in gastric cancer tissues compared with matched normal tissue preparations from the same patients. Results are representative of six separate experiments. E: The concentration of phospho-IGF-IR in gastric cancer tissues as determined by ELISA was significantly higher (24.64 ± 0.1315 U/ml, P < 0.001) compared with that in normal tissues (8.994 ± 0.0904 U/ml). The bar graph is representative of six separate experiments. H: Representative example of mucosa from patients with gastric cancer showing higher expression of phospho-AKT than normal mucosa (×100) (G). J: Intense positive nuclear staining for phospho-AKT as indicated by arrows in the mucosal epithelial cells of the malignant glands (×300). C and I: Bar graphs represent percentage area of phospho-IGF-IR and phospho-AKT-positive cells, respectively *P < 0.05.