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. 2010 Dec;177(6):2860–2869. doi: 10.2353/ajpath.2010.100509

Figure 5.

Figure 5

FGFR4 expression and 5′ DNA methylation in clonal breast carcinoma cell lines. A: Total cell lysates from a panel of human breast carcinoma cell lines were examined by western blotting for FGFR4 expression. B: Examination of 5′ DNA methylation in these cell lines was performed by COBRA as detailed in Materials and Methods. The first lane is a ladder marker, the second (−) is a negative control without BstU1 digestion, and M represents a positive methylation control. This examination identifies the most significant degree of methylation in MCF-7 cells. C: FGFR4 mRNA expression in MCF-7, MDA-231, and MDA-453 cells before (−) and following (+) exposure to 5′azacytidine (AZC) at the indicated concentrations. Note the selective induction in MCF-7 cells. D: COBRA examination of 5′ DNA methylation of corresponding treatments from C confirms selective methylation inhibition in MCF-7 cells.