Table 2.

FGFR4 Primers and PCR Conditions

Primer	Forward	Reverse	Tm (°C)	Product (bp)
RT-PCR and qPCR
FGFR4	5’-ACAGGCTGGTGCTTGGGAAG-3’	5’-GTCCTTGTCAGAGGCGTTGTC-3’	59	146
GAPDH	5’-CTCAGACACCATGGGGAAGG-3’	5’-CAATGAAGGGGTCATTGATGG-3’	59	125
Genotyping and mutational analysis
Codon 388	5’-GGCCAGGTATACGGACATCATCC-3’	5’-AGAGGGAAGCGGGAGAGCTTCTG-3’	61	150
Codon 367	5’-AGAGGAGGACCCCACATGGAC-3’	5’-GGGAGAGCTTCTGCACAGTGG-3’	61	176
Codons 535/576	5’-GATCGGCCGACACAAGAACATC-3’	5’-GAGACCAGGACTGGGAAGGAGAG-3’	61	287
LOH
D5S2111	5’-CATGGAGACAAGCAGACTGTACC-3’	5’-AATGTGAACGAGAGGGGAAAGAG-3’	58	136
D5S469	5’-AATACACGGAATGTGGTCTAGAC-3’	5’-ATTTTACTGAGCATATGTTGAACG-3’	56	133
COBRA
BS (outer)	5’-GGGTGGGATAGGAGGTGGGT-3’	5’-CCTAACTCCTCCTCACCTAACTCCTC-3’	60	168
BS (inner)	5’-GGGTGGGATAGGAGGTGGGT-3’	5’-CCTCACTCCCAACTCCAACC-3’	58	138