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. 2010 Dec;177(6):2886–2897. doi: 10.2353/ajpath.2010.100303

Figure 2.

Figure 2

Effect of MEK1/2 inhibitor U0126 on MDA-MB-231 cell phase distribution and migration. Cells were quiescent for 24 hours before serum stimulation in the absence or presence of 20 μmol/L U0126 and monitored at the indicated times for changes in phospho-ERK1/2 expression, cell phase distribution, and migration. Control is DMSO-treated cells. Blots are representative, and histograms and migration plots are averaged from at least three independent experiments. A: Short-term phospho-ERK1/2 expression by Western blot. Densitometric analysis of phospho-ERK1/2 bands was corrected with the values of respective total ERK1/2 bands and actin. B: Phospho-ERK1/2 expression after inhibitor treatment by Western blot. C: Cell migration analyzed by Boyden chamber assay. ***P < 0.001, two-tailed Student’s t-test. D: Cell phase distribution analyzed by flow cytometry. SM, serum medium; SFM, serum-free medium.