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. 2010 Dec;177(6):3125–3132. doi: 10.2353/ajpath.2010.100369

Figure 1.

Figure 1

Nf1−/− SCCM significantly promotes Nf1+/− mast cell degranulation. Degranulation of mast cells was assessed by the release of β-hexosaminidase. β-Hexosaminidase activity is measured in the supernatant and the extent of degranulation is reported as a percentage of total cellular β-hexosaminidase activity. A: Degranulation of WT and Nf1+/− BMMCs was assessed by the release of β-hexosaminidase after SCCM stimulation. *P < 0.01 for WT versus Nf1−/− SCCM. **P < 0.01 for Nf1+/− versus WT mast cells stimulated with Nf1−/− SCCM. Data are means ± SEM from triplicate samples in four independent experiments. B: β-Hexosaminidase release was measured after stimulation with either Kit-L plus DNP or SCCM (WT or Nf1−/−) with or without the addition of Ack. *P < 0.01 for WT versus Nf1+/− mast cells stimulated with Kit-L/DNP. **P < 0.01 for Nf1+/− versus WT mast cells stimulated with Nf1−/− SCCM and Nf1+/− treated with Ack versus Nf1+/− treated with vehicle. Data are means ± SEM from triplicate samples in four independent experiments. C: The Nf1−/− SCCM promotes mast cell degranulation via the c-Kit receptor. Degranulation of BMMCs derived from WT, Nf1+/−, W41/W41, and Nf1+/−;W41/W41 mice was assessed by the release of β-hexosaminidase after stimulation with Nf1−/− Schwann cell-conditioned medium. *P < 0.01 for WT versus Nf1+/− mast cells stimulated with Nf1−/− SCCM. **P < 0.01 for WT versus W41/W41 mast cells stimulated with Nf1−/− SCCM. ***P < 0.01 for Nf1+/− versus Nf1+/−;W41/W41 mast cells stimulated with Nf1−/− SCCM. Data are means ± SEM from triplicate samples in four independent experiments.