FIG. 5.

Immunofluorescent staining of frozen pancreatic sections from NOD and NOD-Alox15^null islets. Representative sections from 9.5-week-old NOD (A) or NOD-Alox15^null (B) mice, and ≥30-week-old NOD (C) or NOD-Alox15^null (D) mice were stained with antibodies against insulin (red) and CD4 (green), as well as Hoechst dye (blue, nuclear staining). Panels E (NOD) and F (NOD-Alox15^null) show representative sections from ≥30-week-old mice costained for insulin (green), Foxp3 (red), and Hoechst (blue).