Fig. 1.

HFS cells are resistant to cell death induced by tubacin cultured in combination with SAHA, etoposide, or doxorubicin. (A) Western blot analysis probing with antibodies against acetylated α-tubulin and acetylated histone H3 in HFS cells cultured for 24 h with tubacin, SAHA, and tubacin plus SAHA. α-Tubulin and histone H3 are shown as loading controls. Cell growth (B) and viability (C) of HFS cells cultured with tubacin. Cell viability in HFS cells cultured with tubacin in combination with SAHA (D), etoposide (E), or doxorubicin (F). Error bars represent the SD of data points generated from experiments, which were all done in triplicate.