Fig. 6.

Tubacin up-regulates DDIT3 and DDIT4, down-regulates replication proteins, and induces a G1 arrest. (A) Real-time PCR analysis on LNCaP cells cultured with tubacin, SAHA, etoposide, and the combinations of tubacin with SAHA or etoposide for 24 h. Primers used were against DDIT3 and DDIT4. (B) Western blot analysis probing with antibodies against acetylated α-tubulin and DDIT3. GAPDH is shown as a loading control. (C) Real-time PCR analysis on LNCaP cells cultured as described in A. Primers used were against Mcm4, Mcm6, Cdt1, and Psf2. (D) Cells cultured for 24 h as described in A were stained with propidium iodide and assessed by flow cytometry.