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. 2010 Oct 26;107(46):20069–20074. doi: 10.1073/pnas.1007152107

Fig. 3.

Fig. 3.

Template requirements for polymerase binding. (A) RNA probes used to analyze template discrimination. (B) Labeled 3′ RNA probes were incubated with L in the presence of yeast tRNA, and complexes were separated and visualized as in Fig. 2B. (C) Resulting L–RNA complexes formed with a saturated mutagenesis panel of the first seven positions of the 19-nt 3′ RNA probe. RNA binding was quantified and graphed as in Fig. 2C and normalized as indicated.