Table 3.
Polymorphism detection in P. vivax drug resistance genes by microarray and sequencing analysis
| Gene | Chromosome | Microarray polymorphic range (bp) (prediction) | P value cutoff | Illumina sequencing (SNP reads/total reads) |
| pvdhfr | 5 | 964,772–964,814 (964,797) | 10−15 | T964796C [Y69Y] (13/13) |
| 964,742–964,820 (964,797) | 10−10 | C964763G [S58R] (31/31) | ||
| pvdhps | 14 | 1,258,370–1,258,412 (1,258,389) | 10−15 | C1258389T [M205I] (12/12) |
| pvmdr1 | 10 | 363,008–363,055 (363,031) | 10−15 | G363032A [L1022L] (12/13) |
| 363,200–363,242 (363,221) | 10−15 | G363223A [T958M] (18/19) | ||
| 363,362–363,404 (363,383) | 10−05 | T363374G [M908L] (14/15) | ||
| pvcrt | 1 | 330,470–330,518 (330,489) | 10−15 | T330482C [5′ upstream region] (2/2) |
| 330,470–330,518 (330,489) | 10−15 | G330484T [5′ upstream region] (1/1) | ||
| 330,470–330,518 (330,489) | 10−15 | C330495A [5′ upstream region] (4/5) | ||
| 331,138–331,172 (331,152) | 10−12 | T331151C [intronic] (58/58) | ||
| pvmrp | 2 | 154,052–154,088 (154,069) | 10−15 | C154067T [H1586Y] (13/13) |
| 154376–154,412 (154,393) | 10−15 | G154391A [V1478I] (6/6) | ||
| 154,556–154,592 (154,575) | 10−15 | G154567C [G1419A] (8/8) | ||
| 154,628–154,670 (154,647) | 10−15 | T154646G [Y1393D] (11/11) | ||
| 154,964–155,000 (154,983) | 10−15 | T154979A [L1282I] (10/10) |
Microarray polymorphisms are presented as base-pair ranges with the predicted SNP position in parentheses. p value indicates the cutoff for detection of polymorphisms by microarray. Sequencing polymorphisms are presented as SNP base-pair position and nucleotide change. For sequencing results, the predicted amino acid change resulting from the SNP is indicated in brackets.