Fig. 3.

NAADP-AM promotes skeletal muscle differentiation. C2C12 cells were induced to differentiate in the presence or absence of 50 nM NAADP-AM. (A) RNA was harvested at the indicated time points and analyzed for expression of myogenin and skNAC by Northern blot using specific probes. (B) Following 4 d of differentiation, cells were stained for myosin heavy chain and DAPI to determine the differentiation index (percent nuclei in myosin heavy chain-positive cells). (C) Bar chart (mean with SEM; n = 4) representing the differentiation index following treatment for 4 d with either DMSO or NAADP-AM. (D) Primary murine myoblast were induced to differentiate in the presence or absence of 10, 50, 100, or 250 nM NAADP-AM. RNA was harvested at the indicated time points and analyzed for the expression of myogenin and skNAC by Northern blot analysis.