Fig. 2.

Time- and concentration-dependent inactivation of CYP2E1 by DDC. Reconstituted CYP2E1 was incubated with 0 (●), 5(■), 10 (▴), 20 (▾) 40 (♦), 200 (○), and 400 (□) μM DDC. Aliquots were removed at the times indicated and assayed for residual activity as described under Materials and Methods. The inset shows the double reciprocal plot for the initial rates of inactivation as a function of the concentrations of DDC. The kinetic constants were determined from the double reciprocal plot. The data shown represent the average of four separate experiments done in duplicate.